The aim of this study was to determine the effect of anthocyanin from roselle (Hibiscus sabdariffa) supplemented diets on growth, survival and body composition of rainbow trout (Oncorhynchus mykiss) for 60 days. Fish were fed 0 % (control), 0.5 %, 1 % and 2 % anthocyanin extract in complete random design. Fifteen fish with the same initial weight (14 ± 0. 1 g) were selected and randomly distributed into experimental treatments in triplicates and were fed 4 % biomass. Growth performance, feed efficiency, survival and body composition were assed at the end of the rearing period. The result showed that there were no significant difference between the control and anthocyanin treatments in final weight, weight gain (%), specific growth rate (SGR % day-1), protein efficiency ratio (PER), feed conversion ratio (FCR) and feed intake (% day-1), although, growth performance and feed efficiency were higher in anthocyanin 0.5% compared to the other treatments. Morever, There was no significant difference in survival rate between the control and anthocyanin treatments. No significant differences was observed in nutritional carcass between the control and anthocyanin supplement. No significant differences was observed in AST, ALP and LDH between anthocyanin treatments and control group, although ALT significantly increased in 1% anthocyanin extract.The present study demonstrates that anthocyanins from roselle has no effect on growth performance, feed efficiency and body composition of rainbow trout.