Synbiotics and rare elements such as selenium strengthen non-specific defense in aquaculture industry and improve digestion and absorption of diets. The aim of this research was to determine the effect of diet supplemented with organic selenium (Selenomethionine) and synbiotic Dipro+ separately and combined on digestive enzyme activities, intestinal bacterial flora and intestinal histology in Salmo caspius. In this research, 540 pieces of Caspian Sea with an average weight of 66.34 ± 1.45 grams were prepared. After adapting to the experimental conditions, the fish were randomly distributed in 27 fish ponds. This experiment was conducted in the form of a completely randomized design under 8 experimental treatments and a control group (each with 3 repetitions) for 8 weeks. Experimental treatments include: 2 grams of synbiotics per kilogram of diet (treatment 1), 3 grams of synbiotics per kilogram of diet (treatment 2), 2 milligrams of organic selenium per kilogram of diet (treatment 3), 4 milligrams of synbiotics biotic per kg of diet (treatment 4), 2 grams of synbiotic and 2 mg of organic selenium per kg of diet (treatment 5), 2 grams of synbiotic and 4 mg of organic selenium per kg of diet (treatment 6), 3 gram of synbiotic and 2 mg of organic selenium per kg of diet (treatment 7), 3 grams of synbiotic and 4 mg of organic selenium per kg of diet (treatment 8). Then, at the end of the experiment, in order to examine digestive enzyme activities, blood was taken with the an insulin syringe from the caudal artery or vena cava or by cutting the caudal stem. Also, in order to measure intestinal tissue changes, samples were taken from intestine. The highest amount of digestive enzymes, including: amylase, lipase and protease, was recorded in treatment 1, that is, the group fed with 2 grams of synbiotic dipro+, which was significantly different from the control group (P < 0.05). The lowest amount of lactic acid bacteria (LAB) and the total number of intestinal bacteria (TVC) were significantly present in the control group (P < 0.05). The results showed that there was a significant difference between the intestinal tissue indices in the groups fed with nutritional supplements combined and separately with the control group. Altogether, the results showed that the combined use of synbiotic dipro+ and organic selenium could reduce tissue damage and is recommended as a supplement in the diet of Salmo caspius .
Akbarabadi M, Khara H, Vahabzadeh H, Ahmadnezhad M, safari R. The effect of diet supplemented with organic selenium (Selenomethionine) and synbiotic diproplus (Dipro+) separately and combined on the activity of digestive enzymes, intestinal bacterial flora and intestinal histology in Caspian Sea salmon (Salmo caspius). JAIR 2024; 12 (1) :41-50 URL: http://jair.gonbad.ac.ir/article-1-902-en.html