In this study, in order to produce all female Rainbow trout, at first, sperm was harvested and then was irradiated by gamma radiation emitted from co60, b doses of 450, 600, 750, 900 and 1050 Gy. Then the different dozes of sperm was fertilized with normal eggs and thermal shock (28±0.5°C) was used for the induction of diploid. At the next stage for determination of hematological, biochemical and immunological indices, the experimental groups were underwent a six-month training period. At the end of the breeding period blood sampling was taken and then hematological parameters (containing red blood cells, white blood cells, hematocrit and hemoglobin, MCV, MCH and MCHC), biochemical indices (cholesterol, triglycerides, and albumin) and immunological parameters (containing total including protein, lysozyme and immunoglobulin) were measured in different units. For the completion of immune studies (MMCs and accumulation of lymphocytes), anterior sections of kidney tissue were separated and sampled in the experimental groups. The results showed that using gamma radiation for gynogenesis of rainbow trout does not have any significant effect on hematology and immunology indices in rainbow trout at different groups. This is while differentiation in hematological indices (containing red blood cells (RBC), white blood cells (WBC), hematocrit, hemoglobin, MCV, MCH and MCHC) showed significant difference between triploid group and other groups. The result showed that this method of genetic manipulation is comfortable for gynogenesis of this fish specieswithout affecting hematology and immunology indices.