Journal of Applied Ichthyological Research
نشریه علمی پژوهشی پژوهشهای ماهی شناسی کاربردی
JAIR
Agriculture
http://jair.gonbad.ac.ir
1
admin
2423-6349
2423-6349
8
10.22034/jair
14
8888
13
fa
jalali
1404
1
1
gregorian
2025
4
1
13
1
online
1
fulltext
fa
بهینه سازی روش پنوماتیک استحصال تخم در ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss) با تأکید بر بهبود کارایی و نتایج تولیدمثلی
Optimization of Pneumatic Egg Stripping Method in Rainbow Trout (Oncorhynchus mykiss) with Emphasis on Improving Efficiency and Reproductive performance
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">استحصال تخم از مراحل حیاتی در تکثیر ماهی قزل آلای رنگین کمان محسوب می شود. روش سنتی ماساژ دستی با خطراتی مانند تغییر</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> pH </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">مایع تخمدان و آسیب فیزیکی به مولدین همراه است. این مطالعه بهینه سازی روش پنوماتیک را به عنوان جایگزینی کارآمد بررسی کرده و تأثیر نوع گاز ، دبی جریان ، فشار و مشخصات نیدل را ارزیابی نموده است. از </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">8۰</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">قطعه مولد ماده </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۴</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black"> ساله با میانیگن وزنی 0.0035 </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black"> 3783.5 گرم در گروه های آزمایشی استفاده شد. نرخ بازماندگی، مدت استحصال، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">pH </span></span></span></span><span lang="AR-SA" style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">مایع تخمدان، درصد لقاح</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">،</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">چشم زدگی و تفریخ اندازه گیری شدند. روش پنوماتیک با دبی </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۱.۵</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black"> لیتر/دقیقه، بازماندگی مشابه روش دستی (</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۸۵-۹۰</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">٪</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p>0.05</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">را نشان داد، درحالیکه وزن تخم استحصالی به طور معنی داری افزایش یافت ( </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۲۱.۳</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۴۹۷.۱</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">گرم در مقابل </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۱۸.۷ ۳۴۵.۰ </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black"> گرم،</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> p<0.01</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">). </span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">نوع گاز تأثیر متفاوتی بر شاخص های تولیدمثلی نداشت (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p>0.05</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">). این روش زمان استحصال را </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۳۲</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">٪</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black"> کاهش داد (</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۵.۲</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۳۹.۸ </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">ثانیه در مقابل </span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">۶.۱</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:black">±</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black"> ۴۶.۰</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">ثانیه، </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p>0.05</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span 2="" nazanin="" style="font-family:"><span style="color:black">) و تمام تخم ها را از محوطه شکمی خارج نمود. یافته های آزمایش روش پنوماتیک را به عنوان تکنیکی استاندارد و مستقل از اپراتور معرفی میکند که ضمن افزایش بهره وری مراکز تکثیر، رفاه ماهی را به دلیل عدم دستکاری فیزیکی سطح بدن ماهی و بیهوشی مجدد بهبود می بخشد</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:red">.</span></span></span></span><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"></span></span></span></b></span></span></span></span></span><br>
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<span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Egg collection is a critical stage in rainbow trout</span></span></span><i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> (Oncorhynchus mykiss)</span></span></span></i><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> propagation. Traditional manual stripping poses risks including altered ovarian fluid pH and physical injury to broodstock. This study optimized pneumatic extraction as an efficient alternative, evaluating the effects of gas type, flow rate, pressure, and needle specifications. Eighty 4-year-old female broodstock with a mean weight of 3783.5 ± 0.0035 g were allocated to experimental groups. Survival rate, stripping duration, ovarian fluid pH, fertilization rate, eyed egg stage, and hatching rate were measured. Pneumatic extraction at 1.5 L/min flow rate showed comparable survival to manual stripping (85-90%, p>0.05), while significantly increasing egg yield (497.1 ± 21.3 g vs. 345.0 ± 18.7 g, p<0.01). Gas type had no differential effect on reproductive indices (p>0.05). The method reduced stripping time by 32% (39.8 ± 5.2 s vs. 46.0 ± 6.1 s, p>0.05) and achieved complete abdominal evacuation. Findings establish pneumatic extraction as a standardized and operator-independent technique that enhances hatchery productivity while improving fish welfare by body surface manipulation and repeated anesthesia<b>.</b></span></span></span></span></span></span>
قزل آلای رنگین کمان, کیفیت گامتها, تکثیر مصنوعی, استحصال مکانیکی
Oncorhynchus mykiss, Gamete quality, Artificial propagation, Mechanical stripping
51
60
http://jair.gonbad.ac.ir/browse.php?a_code=A-10-1129-1&slc_lang=fa&sid=1
Roghayeh
Mahmoudi
رقیه
محمودی
roghaye.mahmodi@gmail.com
10031947532846009222
10031947532846009222
Yes
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization
سازمان تحقیقات آموزش و ترویج کشاورزی- موسسه تحقیقات علوم شیلاتی کشور-مرکز تحقیقات ژنتیک و اصلاح نژاد ماهیان سردابی یاسوج