Journal of Applied Ichthyological Research
نشریه علمی پژوهشی پژوهشهای ماهی شناسی کاربردی
JAIR
Agriculture
http://jair.gonbad.ac.ir
1
admin
2423-6349
2423-6349
8
10.22034/jair
14
8888
13
fa
jalali
1402
6
1
gregorian
2023
9
1
11
3
online
1
fulltext
fa
تأثیر آفتکش کلرپیریفوس بر ماهی تیلاپیا (Oreochromis niloticus) مقاوم شده با پربیوتیک قارچ صدفی (Pleurotus ostreaus)
The effect of chlorpyrifos pesticide on tilapia (Oreochromis niloticus) resistant to mushroom (Pleurotus ostreaus) probiotic
تخصصي
Special
كاربردي
Applicable
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">وجود آلاینده ­ها در اکوسیستم­های آبی سبب آسیب­های بافتی در ماهیان می­شود. در</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بعضی</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">موارد</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">اثرات</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">تخریب آفتکشها روی</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">موجودات</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">غیر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">هدف (آبزیان)</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">نسبت</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">به</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">موجودات</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">هدف (آفات)</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بیشتر است که این</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">خود</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">سبب مرگومیر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">سریعتر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">و بیشتر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">آبزیان میشود،</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">از این­رو</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">استفاده</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">از</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">محرکهای</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">ایمنی</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بسیار</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">ضروری</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">به نظر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">میرسد. هدف این مطالعه بررسی تأثیر سطوح مختلف پره­بیوتیک قارچ صدفی (</span></span></b><b><i><span dir="LTR" style="font-size:9.0pt">Pleurotus ostreatus</span></i></b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">) بر شاخصهای بافتشناسی ماهی تیلاپیا (</span></span></b><b><i><span dir="LTR" style="font-size:9.0pt">Oreochromis</span></i></b><b><span dir="LTR" style="font-size:9.0pt"> <i>niloticus</i></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">) مواجهه شده با سم کلرپیریفوس بود. این تحقیق در پاییز 1397 انجام شد.</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">به همین منظور تعداد</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">120</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بچه(ماهی)</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">تیلاپیا</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">به</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">مدت</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">42</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">روز در 4 تیمار تقسیم شدند. سپس به</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">هرکدام</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">از</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">گروهها</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">غلظت 5/0 </span></span></b><b><span dir="LTR" style="font-size:9.0pt">ppm</span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">سم کلرپیریفوس به</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">مدت</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">16</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">روز</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">اضافه</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">شد. در پایان دوره ماهیان</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">توسط</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">محلول بیهوش</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">کننده</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">گل</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">میخک (220</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">میلیگرم</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">لیتر)</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بیهوش</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">شده و</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بافت</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">کبد و</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">آبشش آنها</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">برای</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">مطالعات</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">بافتشناسی</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">جدا</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">گردید. نتایج</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">نشان</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">داد</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">تیمارهایی</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">که</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">در معرض 5/0 </span></span></b><b><span dir="LTR" style="font-size:9.0pt">ppm</span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">سم کلرپیریفوس (شاهد سم زده و بدون پره­بیوتیک) بودند. بیشترین</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">اثر</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">تخریب</span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">را در بافت کبد و آبشش داشتند. ولی تیمارهای ترکیبی پره­بیوتیک قارچ صدفی و سم کلرپیریفوس منجر به بهبود آسیب ساختاری بافت ها شدند که نشاندهنده سمزدایی است و همچنین غلظت 2/0 </span></span></b><b> </b><b><span dir="LTR" style="font-size:9.0pt">ppt</span></b><b><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Nazanin"">قارچ صدفی بهترین عملکرد را در برابر آسیب­های بافتی کبد و آبشش ماهی تیلاپیا داشت که نشان دهنده بهبود مقاومت بافتی بود.</span></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-size:10.0pt">Contaminants in aquatic ecosystems cause tissue damage in fish. In some cases, the effects of pesticide degradation on non-target organisms (aquatic) are greater than on target organisms (pests), which in turn causes faster and more aquatic deaths. The use of safety stimuli seems to be essential. The aim of this study was to investigate the effect of different prebiotic levels of <i>Pleurotus ostreatus</i> on histological characteristics of tilapia (<i>Oreochromis niloticus</i>) exposed to chlorpyrifos. This research was conducted in the fall of 2019. For this purpose, 120 tilapia fry were divided into 4 treatments for 42 days. Then, 0.5 ppm concentration of chlorpyrifos was added to each group for 16 days. At the end of the period, the fish were anesthetized with clove anesthetic solution (220 mg / l) and their liver tissue and gills were isolated for histological studies</span><span dir="RTL" lang="FA" style="font-size:10.0pt">. </span><span style="font-size:10.0pt">The results showed that the treatments exposed to 0.5 ppm chlorpyrifos (poisoned control without prebiotics) had the most destructive effect on liver and gill tissue. However, combined prebiotic treatments of Pleurotus ostreatus and chlorpyrifos improved tissue damage, indicating detoxification, and a concentration of 0.2 ppt Pleurotus ostreatus had the best performance against tissue damage to the liver and gills of tilapia, indicating improved resistance. </span><span lang="FA" dir="RTL" style="font-size:10.0pt"></span></span></span></span></span><br>
پربیوتیک, ماهی تیلاپیا, آسیب بافتی, سم کلرپیریفوس
Prebiotic, Tissue damage, Tilapia, chlorpyrifos.
33
42
http://jair.gonbad.ac.ir/browse.php?a_code=A-10-50-7&slc_lang=fa&sid=1
Seyed Ali Akbar
Hedayati
سیدعلی اکبر
هدایتی
hedayati@gau.ac.ir
10031947532846008259
10031947532846008259
Yes
دانشگاه علومکشاورزی و منابع طبیعی گرگان
Somaye
Namroodi
سمیه
نمرودی
hedayati@gau.ac.ir
10031947532846008260
10031947532846008260
No
دانشگاه علومکشاورزی و منابع طبیعی گرگان
Vahid
Zamani
وحید
زمانی
hedayati@gau.ac.ir
10031947532846008261
10031947532846008261
No
دانشگاه کردستان
Rabee
Ziaee
رابعه
ضیائی
hedayati@gau.ac.ir
10031947532846008262
10031947532846008262
No
اداره کل شیلات استان گلستان
Farahnaz
Kakavand
فرحناز
کاکاوند
hedayati@gau.ac.ir
10031947532846008263
10031947532846008263
No
دانشگاه علومکشاورزی و منابع طبیعی گرگان
Atefe
Iri
عاطفه
ایری
hedayati@gau.ac.ir
10031947532846008264
10031947532846008264
No
دانشگاه علومکشاورزی و منابع طبیعی گرگان
Mohammad
Akhavan Bahabadi
محمد
اخوان بهابادی
hedayati@gau.ac.ir
10031947532846008265
10031947532846008265
No
مرکز تحقیقات ملی آبزیان آبهای شور بافق کرمان
Maryam
Rezaei Shadgan
مریم
رضایی شادگان
hedayati@gau.ac.ir
10031947532846008266
10031947532846008266
No
دانشگاه علومکشاورزی و منابع طبیعی گرگان