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The intensity of electrophoretic bands containing egg non-vitellogenin derived proteins in relationship with embryo-larvae viability in common dentex (Dentex dentex) a preliminary evaluation
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Mohammadreza Samaee *   |
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Abstract: (1504 Views) |
| Despite the reported effects of egg vitellogenin-derived proteins (VtgDP) and amino acids on embryo-larvae viability in common dentex the effects of non-vitellogenin-derived proteins (non-Vtg-DP) has yet to be determined. As an initial study, fertilized eggs (70 batches) were provided by natural spawning of broodfish in captivity. Viability parameters (VP) such as egg floating, hatching, and larval survival rate were calculated. Egg batches were categorized as low, medium, and high quality upon hatching rate. Based on the SDS-PAGE of egg crude extracts 63 protein bands (16.7 to 131.4 kDa) were identified. The intensity of bands on gel (densitometry; <200 to 877 μg mL-1), and their biochemical nature (specific staining) was determined. After subjecting the bands to a screening process, 11 bands were considered as non-vtgDP-contained bands. The highest intensity of the bands was observed in the gels belonging to high quality eggs. The intensity of 9 bands of 11 (individually or in combination with other protein bands) was significantly (p<0.001) and positively correlated to VPs via a series of simple and multiple regression models. In brief, the preliminary results revealed the importance of the class of egg proteins and their effects (individual and combined) on the viability of common dentex larvae and indicated their probable potential use to predict egg quality in teleosts. It also showed that it is worth to conduct a deeper study for more detailed characterization of the protein content of the selected bands and to evaluate the mechanism of their effects on larval success |
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| Keywords: Egg quality, marine fish species, bio-markers |
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Full-Text [PDF 799 kb]
(321 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2021/10/22 | Accepted: 2021/11/22 | Published: 2021/12/17
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