:: Volume 6, Issue 4 (4-2018) ::
JAIR 2018, 6(4): 109-126 Back to browse issues page
A comparative study of the in vitro antimicrobial and antifungal effect of Trachyspermumammi ,Lavandulaangustifolia and Eugenia aromatica essence on rainbow trout some patoghen
Abstract:   (2993 Views)
Regarding the revealing side effect of chemical medications, such as antimicrobial resistance and etc, herbal medications have been considered as one of potential alternative to chemical one. The main purpose of this study was to evaluate the in vitro antibacterial effect of lavender ( Lavandula angustifolia) and ajwain ( Trachyspermum ammi) essential oil on two important rainbow trout bacteria; streptococcus iniae and Lactococcus garvieae .In addition, antifungal activity of Clove (Eugenia aromatic) and lavender essential oil were assessed as well. Disc diffusion method was used to test antibacterial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by using standard procedure Broth- microdilution. The results obtained demonstrated that Streptococcus iniae and Lactococcus garvieae were most sensitive to ajwain with inhibition zone of respectively 26±1mm & 24.33±1.15mm , which was significantly higher than amoxicillin , erythromycin and lavender (p<0.05). Moreover, ajwan essential oil showed the lowest MIC (0.049±0.01) compared to the other group. Antifungal activity of clove oil and lavender did not show significant difference comparing to Clotrimazol. However, these two herbal agents could not compete with Amphotericin b whith the highest zone of inhibition (13mm) (p<0.05). Therefore clove oil , lavender and ajwain essentialsare suggested as natural anti fungus and anti bacteria agents in aquaculture activities.
Keywords: : essential oil, antibacterial effect, Antifungal effect, rainbow trout, Streptococcus iniae, Lactococcus garvieae
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Type of Study: Applicable | Subject: Special
Received: 2017/10/9 | Accepted: 2017/11/26 | Published: 2019/04/1
References
1. Aberoomand Azar P., Mottaghianpuor Z., Sharifan A., Larijani K. 2010. Studies on the Effect of Extraction Method on Chemical Composition and Antimicrobial Activity of Carum copticum Essential Oil. Food Technology & Nutrition, 7(2): 10-18. (In Persian).
2. Akhlaghi M., Mahjoor M. 2000. Some histopathological aspects of streptococcosis in cultured rainbow trout (Oncorhynchus mykiss). Bulletin of the European Association of Fish Pathologists, 24: 132-136.
3. Andrews J.M. 2001. Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48: 5-16.
4. Austin B., Austin D.A. 2007. Bacterial Fish Pathogens. Disease of Farmed and Wild Fish. Springer Praxis Books. 552 P.
5. Azizkhani M., Tooryan F.2016. Effects of Carum Copticum essential oil on growth and gene expression of enterotoxins A and C in Staphylococcus aureus in Kilka fish (Clupeonella cultriventris caspia) surimi. Journal Fisheries Science & Technology, 5(1): 141-152. (In Persian).
6. Blažeković B., Stanic G., Pepeljnjak S., Pepeljnjak S., Vladimir-Knežević S. 2011.In Vitro Antibacterial and Antifungal Activity of Lavandula ´ intermedia Emeric ex Loisel. ‘Budrovka’. Molecules, 16: 4241-4253.
7. Bouchard L., Patel J., Lahey L. 2000. The effect of clove oil on fungal infections of salmonid eggs. Journal of Special Publication - Aquaculture Association of Canada, 4(110): 81-84.
8. Bown D. 1996. The royal horticultural society encyclopedia herbs their uses. London: Dorling Kindersley Distributed by Houghton Mifflin, 7: 363-364.
9. Bruno D.W., Woo B.P. 1994. Fish Diseases Disorders, Volume 3, Viral, Bacterial and Fungal Infections. In: Woo PTK, Bruno DW (Eds.). CABI Publishing, Waling Ford, Oxon, United Kingdom, pp: 559-599.
10. Bulfon C., Volpatti D., Galeotti M. 2014. In vitro vntibacterial activity of plant ethanolic extracts against fish pathogens. Journal of the World Aquaculture Society, 45: 545-557.
11. Celikel N., Kavas G. 2008. Antimicrobial properties of some essential oils against some pathogenic microorganisms. Czech Journal of Food Science, 3: 174-181.
12. Chaieb K., Zmantar T., Ksouri R., Hajlaoui H., Mahdouani K. Abdelly C., Bakhrouf A. 2007. Antioxidant properties of the essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species. Journal of Mycoses, 50: 403-406.
13. Chami F., Chami N., Bennis S., Trouillas J., Remmal A. 2004. Evaluation of carvacrol and eugenol as prophylaxis and treatment of vaginal candidiasis in an immunosuppressed rat model. Journal of Antimicrobial Chemother, 54: 909-914.
14. Costa G., Danz H., Kataria P., Bromage E. 2012. A holistic view of the dynamisms of teleost IgM: A case study of Streptococcus iniae vaccinated rainbow trout (Oncorhynchus mykiss). Development Competition Immunology, 36: 298-305.
15. Cruz T., Cabo M.M., Castillo M.J., Jimenez J., Ruiz C., Ramos-Cormenzana A. 2006. Chemical composition and antimicrobial activity of the essential oils of different samples of Thymus baeticus boiss. Phototherapy Research, 7(1): 92-94.
16. Devasankaraiah G., Hanin I., Haranath P.S., Ramanamurthy P.S. 1974. Cholinomimetic effects of aqueous extracts from Carum copticum seeds. British Journal of Pharmacology, 52: 613-614.
17. Dufour A., Snozzi M., Koster W., Bartram J., Ronchi E. 2003. Assessing Microbial Safety of Drinking Water: Improving Approaches and Methods. World Health Organization. Geneva, Switzerland. 295 P.
18. Dügenci S.K., Arda N., Candan A. 2003. Some medicinal plants as immunostimulant for fish. Journal of Ethno Pharmacology, 88: 99-106.
19. Fereidouni M., Akhlaghi M. 2009. Resistance pattern of Streptococcus iniae to antibiotics in rainbow trout. The first international congress on aquatic animal health management and disease, Tehran Iran. Vibrio parahaemolyticus. International Journal of Food Microbiology, 111: 6-11. (In Persian).
20. Fereidouni M., Akhlaghi M., Khadem Alhoseini A. 2013. Antibacterial effects of medical plant extracts against Lactococcus garvieae the etiological agent of rainbow trout Lactococcosis. International Journal of Aquatic Biology, 1(3): 119-124. (In Persian).
21. Forneris G., Bellardi S., Palmegiano G.B., Saroglia M., Sicuro B., Gasco L., Zoccarato I. 2003. The use of ozone in trout hatchery to reduce saprolegniasis incidence. Aquaculture, 221: 157-166.
22. Gang-Joon H., Cheol-Hyun K., Se-Chang P., Mahanama De Z., Gee-Wook S.h. 2012. Antimicrobial activity of thymol against pathogenic gram-negative bacteria of fishes. The Philippine Journal of Veterinary Medicine, 49: 103-106.
23. Gayoso C.W., Lima E.O., Oliveira V.T., Pereira F.O., Souza E.L., Lima I.O., Navarro D.F. 2005. Sensitivity of fungi isolated from onychomycosis to Eugenia cariophyllata essential oil and eugenol. Fitoterapia, 76: 247-249.
24. Goudarzi M.A., Hamedi B., Malekpoor F., Abdizadeh R., Ghasemi Pirbalouti A., Raissy M. 2011. Sensitivity of Lactococcus garvieae isolated from rainbow trout to some Iranian medicinal herbs. Journal of Medicinal Plants Research, 5: 3067-3073.(In Persian).
25. Hanamanthagouda M.S., Kakkalameli S.B., Naik P.M., Naik P.M., Nagella P., Seetharamareddy H.R., Murthy H.N. 2010. Essential oils of Lavandula bipinnata and their antimicrobial activities. Food Chemistry, 118: 836-839.
26. Harikrishnan R., Nisha Rani M., Balasundaram C. 2003. Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture, 221: 41-50.
27. Heidari-Sureshjani M., Tabatabaei-Yazdi F., Alizadeh-Behbahani B., Mortazavi A. 2013. Antimicrobial effect of aqueous, ethanol, methanol and glycerin extracts of Satureja bachtiarica on Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis. Zahedan Journal of Research in Medical Sciences, 6:29-33. (In Persian).
28. Hoskonen P., Heikkinen J., Eskelinen P., Pirhonen J. 2013. Efficacy of clove oil and ethanol against Saprolegnia sp. and usability as antifungal agents during incubation of rainbow trout Oncorhynchus mykiss (Walbaum) eggs. Aquaculture Research, 46(3): 581-589.
29. Hosseini H., Rabiei S., Rezaei M., Mousavi T. 2013. Inhibitory effects of Ajowan essential oil on growth of Listeria monocytogenes in Rutilus frissi kutum broth medium and fillet. Journal of Nutrition Sciences & Food Technology, 8(2): 71-80. (In Persian).
30. Hussain T., Arshad M., Khan S., Sattar H., Qureshi M.S. 2011. In vitro screening of methanol plant extracts for their antibacterial activity. Pakistan Journal of Botany, 43: 531-538.
31. Immanuel G., Vincybai V.C., Sivaram V., Palavesam A., Marian M.P. 2004. Effect of butanolic extracts from terrestrial herbs and seaweeds on the survival, growth and pathogen (Vibrio parahaemolyticus) load on shrimp Penaeus indicus juveniles. Aquaculture, 236: 53-65.
32. Jianu C., Pop G., G.T., Horhat F.G. 2013. Chemical composition and antimicrobial activity of essential oils of lavender (Lavandula angustifolia) and lavandin (Lavandula x intermedia) grown in Western Romania. Internatioal Journal of Agriculture and Biology, 15: 772-776.
33. Kim K.J., Kim Y.H., Yu H.H., Jeong S.I., Cha J.D., Kil B.S., You Y.O. 2003. Antibacterial activity and chemical composition of essential oil of Chrysanthemum boreale. Planta Medica, 69: 274-277.
34. Kordali S., Kotan R., Mavi A., Cakir A., Ala A., Yildirim A. 2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry. 53: 9452-9458.
35. Lodhia M.H., Bhatt K.R., Thaker V.S. 2009. Antibacterial activity of essential oils from palmarosa, evening primrose, Lavender and Tuberose. Indian Journal of Pharmaceutical Sciences, 71(2): 134-136.
36. López P., Sánchez C., Batlle R., Nerín C. 2005. Solid and vapour-phase antimicrobial activities of six essential oils: susceptibility of selected foodborne bacterial and fungal strains. Journal of Agricultural and Food Chemistry, 53: 6939-6946.
37. Maberley D.J. 1997. Plant Book. A portable Dictionary of Vascular Plants. 2th ed. Cambridge university press, United Kingdom. 874 P.
38. Meyer F.P., Jorgenson T.A. 1983. Teratological and other effects of malachite green on development in rabbits and rainbow trout. Translation of the American Fisheries Society, 112: 818-814.
39. Najafi Sh., Razavizadeh R., Shafagh M. 2012. Effect of water deficit stress on morphological and physiological indices of Carum copticum. Iranian Journal of Plant Biology, 6(22): 25-38. (In Persian).
40. Noga E.J. 1996. Fish Diseases and Diagnosis and Treatment. Mosby-Year Book, Incorporation, Saint. Louis, Monaco, USA. 367 P.
41. Oroojalian F., Kasra-Kermanshahi R., Azizi M., Bassami M.R. 2010a. Phytochemical composition of the essential oils from three Apiaceae spp. and their antibacterial effects on food-borne pathogens. Food Chemistry, 120:765-70. (In Persian).
42. Oroojalian F., Kasra-Kermanshahi R., Azizi M., Bassami M.R. 2010b. Synergistic antibacterial activity of the essential oils from three medicinal plants against some important food-borne pathogens by microdilution method. Journal of Medicinal and Aromatic Plants, 26: 133-146. (In Persian).
43. Pinto E., Vale-Silva L., Cavaleiro C., Salgueiro L. 2009. Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. Journal of Medical Microbiology, 58(11): 1454-1462.
44. Rani P., Khullar N. 2004. Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi drug resistant Salmonella typhi. Phototherapy Research, 18: 670-673.
45. Ravelo C., Magarinos B., Romalde J.A., Tornazo A.E. 2001. Conventional versus miniaturized system for the phenotypic characterization of Lactococcus garveae strains. Bulletin of the European Association of Fish Pathologists, 21: 136-144
46. Samsam Sheriat H .2006. Medicinal Plants. Chaharbagh publishing Do., Tehran, Iran.212 P.
47. Singh G., Kapoor I.P., Pandey S.K., Singh U.K., Singh R.K. 2002. Studies on essential oil part 10 antibacterial activity of volatile oils of some spices. Phototherapy Research, 16: 680-682.
48. Sivam G.P. 2001. Recent advances on the nutritional effects associated with the use of garlic as supplement. American Society of Nutrition Sciences, 131: 1106-1108.
49. Starliper C.E., Ketola H.G., Noyes A.D., Schill W.B., Henson F.G., Chalupnicki M.A., Dittman D.E. 2015. An investigation of the bactericidal activity of selected essential oils to Aeromonas spp. Journal of Advanced Research, 6: 89-97.
50. Uniyal V., Bhatt R.P., Saxena S., Talwar A. 2012. Antifungal activity of essential oils and their volatile constituents against respiratory tract pathogens causing Aspergilloma and Aspergillosis by gaseous contact. Journal of Applied and Natural Science, 4(1): 65-70.
51. Soković M., Glamočlija J., Marin P.D., Brkić D., van Griensven L.J. 2010. Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model. Molecules, 15: 7532-7546.
52. Wang C.Y., Shie H.S., Chen S.C., Huang J.P., Hsieh I.C., Wen M.S. 2007. Lactococcus garvieae infections in humans: possible association with aquaculture outbreaks. International Journal of Clinical Practice, 61(1): 68-73.
53. Wenqiang G., Shufen L., Ruixiang Y., Shaokun T., Can Q. 2007. Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods. Food Chemistry, 101: 1558-1564.
54. Willoughby S. 1989. The Fishing Industry of Barbados. Government Printing Department. Bridgetown, Barbados, North America. 20 P.
55. Zheng G.Q., Kenney P.M., Lam L.K.T. 1992. Sesquiterpensfrom clove (Eugenia caryophylata) as Potential Anticarcinogenic Agents. Journal of Natural Products, 55 (7): 999-1003.
56. Zuzarte M., Vale-Silva L., Gonçalves M.J., Cavaleiro C., Vaz S., Canhoto J., Salgueiro L. 2012. Antifungal activity of phenolic-rich Lavandula multifida L. essential oil. European Journal of Clinical Microbiology and Infectious Disease, 31(7): 1359-1366.

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