[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing Databases

AWT IMAGE

    www.isc.gov.ir 

..
:: Volume 7, Issue 1 (4-2019) ::
JAIR 2019, 7(1): 101-116 Back to browse issues page
Evaluation of gill metabolites of Iranian sturgeon fingerlings Acipenser persicus at different levels of water salinity using HNMR method
Khosrow Rahimi , Iman Sourinejad *
Abstract:   (3223 Views)
Abstract
In the present study, 180 Persian sturgeon fries at releasing weight with average weight of 1.8 ± 0.6 g were exposed to three different salinity levels including 0 (fresh water), 6 and 12 ppt in 96-hour and 10-day periods for measuring their gill metabolites by H-NMR based metabolomics. The results showed that changes in salinity caused changes in the metabolites involving in osmotic regulation, such as betaine, methionine, and threonine and also changes in energy metabolites such as alanine, DMA, and glutamine. After studying the metabolites, it was found that alanine, betaine, serine, glutamine, threonine, dimethylamine, fumaric acid, methionine, tyrosine, leucine, acetone, isoleucine, serine and formic acid showed significant differences between fresh and 12 ppt water. The metabolites including alanine, betaine, glutamine, threonine, dimethylamine, fumaric acid, methionine, acetic acid, and choline were significantly different between fresh and 6 ppt water, and the metabolites including lactic acid, formic acid, and serine showed significant differences between 6 and 12 ppt water (P<0.05). The need to provide energy and immunity in conditions of increasing salinity caused significant increasing in branched amino acids, lactate and alanine in 12 ppt salinity water in compare to fresh water (P<0.05). Methionine and taurine which are responsible for coping with salinity stress, as well as glutamine and fumaric acid, which change at oxygen stress, also significantly increased (P <0.05) between fresh and 12 ppt salinity water. The betaine metabolite which acts as osmotic regulator in salinity conditions and prevents intracellular water loss, showed a significant reduction (P <0.05) in 12 ppt water in compare to fresh water. The results of this study indicate that fumaric acid, methionine and branched chain amino acids are the most important biomarkers of salinity tolerance in Persian sturgeon fries.
Keywords: Acipenser persicus, Osmoregulation, Stress, Metabolomics
Full-Text [PDF 544 kb]   (739 Downloads)    
Type of Study: Research | Subject: Special
Received: 2019/01/26 | Accepted: 2019/02/17 | Published: 2019/04/25
References
1. Allen P.J., Mitchell Z.A., DeVries R.J., Aboagye D.L., Ciaramella M.A., Ramee S.W., Stewart H.A., Shartau R.B. 2014. Salinity effects on Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus Mitchill, 1815) growth and osmoregulation. Applied Ichthyology, 30: 1229-1236.
2. Aru V., Pisano M.B., Savorani F., Engelsen S.B., Cosentino, S., Cesare Marincola F. 2016. Metabolomics analysis of shucked mussel’s freshness. Food Chemistry, 205: 58-65.
3. Bone Q., Marshall N.B. 1982. Biology of Fishes. Chapman and Hall press. New York, USA. 253 P.
4. Boothroyd M., Whillans T., Wilson C.C. 2017. Translocation as a mitigation tool: Demographic and genetic analysis of a reintroduced lake sturgeon (Acipenser fulvescens Rafinesque, 1817) population. Applied Ichthyology, 34(2): 348-363.
5. Bundy J.G., Davey M.P., Viant M.R. 2009. Environmental metabolomics: a critical review and future perspectives. Metabolomics, 5: 3-21.
6. Bystriansky J.S., Frick N.T., Ballantyne J.S. 2007. Intermediary metabolism of Arctic char Salvelinus alpinus during short-term salinity exposure. Journal of Experimental Biology, 210: 1971-1985.
7. Calder P.C. 2006. Branched-chain amino acids and immunity. Journal of Nutrition,136: 288-293.
8. Chang E.W.Y., Loong A.M., Wong W.P., Chew S.F., Wilson J.M., Ip Y.K. 2007. Changes in tissue free amino acid contents, branchial Na+ /K+ -ATPase activity and bimodal breathing pattern in the freshwater climbing perch, Anabas testudineus (Bloch), during seawater acclimation. Journal of Experimental Zoology, 307: 708-723.
9. Chesley A., Richard A., Howlett G., Heigenhauser J.F., Hultman E., Lawrence L.S. 1998. Regulation of muscle glycogen lytic flux during intense aerobic exercise after caffeine ingestion. American Journal of Physiology, 275(44): 596-603.
10. Choi I.Y., Seaquist E.R., Gruetter R. 2003. Effect of hypoglycemia on brain glycogen metabolism in vivo. Journal of Neuroscience Research, 72: 25-32.
11. Evans D.H., Piermarini P.M., Choe K.P. 2005. The Multifunctional Fish Gill: Dominant Site of Gas exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste. American Physiological Society, 85(1): 97- 177.
12. Fernández-Alacid L., Sanahuja I., OrdóñezGrande B., Sánchez-Nuño S., Herrera M., Ibarz A. 2019. Skin mucus metabolites and cortisol in meagre fed acute stress-attenuating diets: Correlations between plasma and mucus. Aquaculture, 499: 185-194.
13. Finney S.T., Isely J.J., Cooke D.W. 2006. Upstream Migration of Two Pre- Spawning Shortnose Sturgeon Passed Upstream of Pinopolis Dam, Cooper River, South Carolina. Southeastern Naturalist, 5(2): 369-375.
14. Folch J., Less M., Stanley G.H.S. 1975. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226: 497-509.
15. German J.B., Hammock B.D., Watkins S.M. 2005. Metabolomics: building on a century of biochemistry to guide human health. Metabolomics, 1: 3-9.
16. Gershanovich A.D., Vaitman G.A., Vladimirsky S.S., Rubtsova T.E. 1991. Changes in chemical composition of muscle in young hybrids between Russian Sturgeon Acipenser guldenstadti Brand×beluga Huso huso. under different levels of salinity. Comparative Biochemistry and Physiology, 100: 667-73.
17. Hajirezaee S., Mirvaghefi A.R., Farahmand H., Agh N. 2018. A metabolic approach to understanding adaptation to sea water by endangered Persian sturgeon, Acipenser persicus Fingerlings. Aquaculture Research, 49: 341-351.
18. Hendry C., Haxton T., Friday M., Cano T. 2015. Assessing the Magnitude of Effect of Hydroelectric Production on Lake Sturgeon Abundance in Ontario. North American Journal of Fisheries Management, 35(5): 930-941.
19. Hwang P.P., Lee T.H. 2007. New insights into fish ion regulation and mitochondrion-rich cells. Comparative Biochemistry and Physiology, 148: 479- 497.
20. Kultz D., Jress D. 1993. Biochemical characterization of isolated branchial mitochondria-rich cells of Oreochromis mossambicus acclimated to fresh water or hyperhaline sea water. Comparative Biochemistry and Physiology, 163(5):406-412.
21. Lankadurai B.P., Nagato E.G., Simpson M.J. 2013. Environmental metabolomics: An emerging approach to study organism responses to environmental stressors. Environmental Reviews, 21(3): 180-205.
22. Marshall W.S. 2002. Na+, Cl−, Ca2+ and Zn2+ transport by fish gills: retrospective review and prospective synthesis. Journal of Experimental Zoology, 293: 264-283.
23. Mommsen T.P., French C.J., Hochachka P.W. 2011. Sites and patterns of protein and amino acid utilization during the spawning migration of salmon. Canadian Journal of Zoology, 58(10): 1785-1799.
24. Rito J., Viegas I., Pardal M.A., Metón I., Baanante I.V., Jones J.G. 2019. Utilization of glycerol for endogenous glucose and glycogen synthesis in sea bass (Dicentrarchus labrax): A potential mechanism for sparing amino acid catabolism in carnivorous fish. Aquaculture, 498: 488-495.
25. Sadok S., Hetli M., El Abed A., Uglow R.F. 2004. Changes in some nitrogenous compounds in the blood and tissues of freshwater pikeperch (Sander lucioperca) during salinity acclimation. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 138: 9-15.
26. Sangiao-Alvarellos S., Laiz-Carrión R., Guzmán J.M., Martín del Río M.P., Miguez J.M., Mancera J.M., Soengas J.L. 2003. Acclimation of S. aurata to various salinities alters energy metabolism of osmoregulatory and nonosmoregulatory organs. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 285: 897-907.
27. Schneider S.M., Joly F., Gehrardt M.F., Badran A.M., Myara A., Thuillier F., Coudray-Lucas C., Cynober L., Trivin F., Messing B. 2006. Taurine status and response to intravenous taurine supplementation in adults with short-bowel syndrome undergoing long-term parenteral nutrition: a pilot study. British Journal of Nutrition, 96(2): 365-70.
28. Schreck C.B., Tort L., Farrell A.P., Brauner C.J. 2016. Biology of Stress in Fish. The Concept of Stress in Fish. In Fish Physiology, Academic Press, (Vol. 35). San Diego, CA, USA. 602 P.
29. Soltan Karimi S., Kalbasi M.M. 2018. Effect of water filters containing silver nanoparticles during incubation on changes in lactate metabolites and glutathione peroxidase and lactate dehydrogenase enzymes in Persian sturgeon embryos Acipenser persicus. Physiology and Aquatic Biotechnology, 6(1): 137- 155. (In Persian)
30. Sreekumar A., Poisson L.M., Rajendiran T.M., Khan A.P., Cao Q., Yu J., Laxman B., Mehra R., Lonigro R.J., Li Y., Nyati M.K., Ahsan A., Kalyana-Sundaram S., Han B., Cao X., Byun J., Omenn G.S., Ghosh D., Pennathur S., Alexander D.C., Berger A., Shuster J.R., Wei J.T., Varambally S., Beecher C., Chinnaiyan A.M. 2009. Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature, 457(7231): 910-914.
31. Subhash Peter M.C., Rejitha V. 2011. Interactive effects of ambient acidity and salinity on thyroid function during acidic and post-acidic acclimation of airbreathing fish (Anabas testudineus Bloch). General and Comparative Endocrinology, 174(2): 175-83.
32. Walton M.J., Cowey C.B. 1977. Aspects of ammoniogenesis in rainbow trout (Salmo gairdneri). Comparative Biochemistry and Physiology, 57: 143-150.
33. Wenwen J., Xiangli T., Ziheng F., Li L., Shuanglin D., Haidong L., Kun Zh. 2019. Metabolic responses in the gills of tongue sole (Cynoglossus semilaevis) exposed to salinity stress using NMR-based metabolomics. Science of the Total Environment, 653: 465-474.
34. Wu H., Liu J., Lu Z., Xu L., Ji C., Wang Q., Zhao J. 2017. Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities. Fish and Shellfish Immunology, 63: 417-423.
35. Wu H., Liu X., Zhang X., Ji C., Zhao J., Yu J. 2013. Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities. Aquatic Toxicology, (136-137): 91-100.
36. Yelamanchi S.D., Jayaram S., Thomas J.K., Gundimeda S., Khan A.A., Singhal A., Keshava Prasad T.S., Pandey A., Somani B.L., Gowda H. 2016. A pathway map of glutamate metabolism. Journal of Cell Communication and Signaling, 10(1):69-75.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahimi K, Sourinejad I. Evaluation of gill metabolites of Iranian sturgeon fingerlings Acipenser persicus at different levels of water salinity using HNMR method. JAIR 2019; 7 (1) :101-116
URL: http://jair.gonbad.ac.ir/article-1-640-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 7, Issue 1 (4-2019) Back to browse issues page
نشریه علمی پژوهشی پژوهشهای ماهی شناسی کاربردی Journal of Applied Ichthyological Research
Persian site map - English site map - Created in 0.06 seconds with 39 queries by YEKTAWEB 4660