[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 7, Issue 2 (7-2019) ::
JAIR 2019, 7(2): 13-28 Back to browse issues page
Study on the activity of some digestive enzymes and biochemical parameters of blood effected by applying Nacl in the diet of farmed Beluga (Huso huso) juveniles
Somayeh Hasanpor Dr, Mahamadreza Ghomi Dr *, Habib Vahabzadeh rodsary Dr, Hamed Mousavi Dr, Rezvan Kazemi Dr
Abstract:   (974 Views)
The recent study carried out to investigate digestive enzymes and blood biochemical parameters in farmed Huso huso juveniles feeding diet containing different levels of Nacl. This experiment includes 4 treatments. The Nacl added in 4 levels of 0, 3, 6 and 9 percent in to the main diet. In this regard 480 species of farmed Huso huso with mean weight of 175g and 5 months old, placed in 12 fiberglass vans having 2000 liter volume for 12 weeks. At the end of experiment, the parameters such as cholesterol, triglyceride, total protein, total fat, glucose, osmolarity, chlorine and sodium, also some digestive enzymes like Alkaline phosphatase, protease, lipase and amylase have been investigated. The results showed that in all treatments, digestive enzymes increased significantly compared to control group (P<0.05) and Alkaline phosphatase indicated the highest increase. So as, in control group it was 3280±405µ/kg and increased to 12117.50±1667.50 µ/kg in diet containing 6% Nacl. The results showed that the rate of sodium and chlorine in fish blood serum was significantly higher in treatment 3, compared to control group, but no significant differences observed in other biochemical parameters between treatment and control group. The results of this study indicated that adding Nacl to the diet can improve function of digestive system, fish growth and health.
Keywords: Beluga (Huso huso), Nacl in the diet, Digestive enzymes and Blood biochemical parameters
Full-Text [PDF 500 kb]   (177 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/10/9 | Accepted: 2018/12/11 | Published: 2019/06/25
1. Akrami R., Ghelichi A., Ahmadifar E. 2011. Effect of dietary prebiotic inulin on hematologi- cal and biochemical parameters of cultured juvenile beluga (Huso huso). Journal of Veterinary Research, 66: 131-136. (In Persian).
2. Appelbaum S., Arockiaraj A.J. 2008. Israeli researchers test viability of using brackish inland waters for rearing gilthead sea bream. Hatchery international, 9(4): 22-23.
3. Appelbaum S., Arockiaraj A.J. 2009. Cultivation of gilthead sea bream (Sparus aurata L.) in low salinity inland brackish geothermal water. AACL Bioflux, 2 (2): 197-203.
4. Asgari R., Rafiee Gh., Eagderi S., Shahrooz R., Pourbagher H., Agh, N., Gisbert E. 2013. Ontogeny of the digestive system in hatchery produced beluga (Huso huso Linnaeus, 1758); a comparative study between beluga and genus Acipenser. Aquaculture Nutrition, 416-417: 33-40.
5. Babaei S.S., Abedian Kenari A., Rajabmohammad Nazari R.M., Gisbert E. 2011. Developmental changes of digestive enzymas in Persian sturgeon (Acipenser persicus) during larval ontogeny. Aquaculture, 318: 138-144.
6. Baqa'i Bambardi M., Faghani Langroudi H., Toloui M., Samii Ardakani M. 2013. The effect of probiotic bectocetal on the biological factors of baby fila (Huso huso). Quarterly Journal of Applied and Aquaculture Sciences, 1(1): 21-34
7. Buddington R.K., Doroshov S.I. 1986. Digestive enzyme complement of white sturgeon (Acipenser transmontanus). Comparative Biochemistry and Physiology, 83: 561-567.
8. Buddington R.K., Krogdahl A. 2004. Hormonal regulation of the fish gastrointestinal tract. Comparative Biochemistry and Physiology, Part A: enzymes in plasma at different measuring temperatures, 38: 555-561.
9. Bullis R.A. 1993. Ctinical Photology of Temprate Freshwater and Estuarine Fishes. In: Stoskopf MK (Eds.). Fish Medicine, Stoskopf, Philadelphia, USA, pp: 232-239.
10. Burr G., Gatlin D., Ricke S. 2005. Microbial ecology of the gastrointestinal tract of fish and the potential application of prebiotics and probiotics in finfish aquaculture. Journal of the World Aquaculture Society, 36(4): 425-436.
11. Cahu C., Zambonino Infante J., Quazuguel P, Le Gall M. 1999. Protein hydrolysate vs. fish meal in compound diets for 10-day old sea bass Dicentrarchus labrax larvae. Aquaculture, 171: 109-119.
12. Carmona R., Domezain A., Garcia-Gallego M., Antonio Hernando J., Rodrigues F., Ruiz-Rejon M. 2009. Biology, Conservation and Sustainable Development of Sturgeons, Springer publication, Netherlands. 476 P.
13. Doumas B.T., Bayse D.D., Carter R.J., Peters T.J.R., Schaffer R.A. 1981. Candidate reference method for determination of total protein in serum. Development and Validation. Clinical Chemistry, 10: 42-50.
14. Eroldoğan O. T., Kumlu M., Aktaş M. 2004. Optimum feeding rate for European sea bass reared in seawater and freshwater. Aquaculture, 231: 501-515.
15. Firouzbakhsh F., Noori F., Khalesi M., Jani-Khalili K. 2011. Effects of a probiotic, protexin, on the growth performance and hematological parameters in the Oscar (Astronotus ocellatus) fingerlings. Journal of Fish Physiology and Biochemistry, 37: 833-842.
16. Furne J., Saeed A., Levitt M.D. 2008. Whole tissue hydrogen sulfide concentrations are orders of magnitude lower than presently accepted values. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 295(5): 1479-1485.
17. García-Meilán I., Valentín J., Fontanillas R., Gallardo M. 2013. Different protein to energy ratio diets for gilthead sea bream (Sparus aurata): Effects on digestive and absorptive processes. Aquaculture, 412: 1-7
18. Ghomi M.R., Shahriari R., Langroudi H.F., Nikoo M., Von Elert E. 2012. Effects of exogenous dietary enzyme on growth, body composition, and fatty acid profiles of cultured great sturgeon Huso huso fingerlings. Aquaculture International, 20: 249-254.
19. Gisbert E., Sarasquete M.C., Williot P., Castello-Orvay F. 1999. Histochemistry of the development of the digestive system of Siberian sturgeon (Acipenser baeri, Brandt) during early ontogeny. Journal of Fish Biology, 55: 596-616.
20. Harpaz S., Hakim T.Y., Slosman T., Eroldoğan O.T. 2005. Effects of adding salt to the diet of Asian sea bass Lates calcarifer reared in fresh or salt water recirculating tanks, on growth and brush border enzyme activity. Aquaculture, 248: 315-324
21. Hidalgo M.C., Urea E., Sanz A. 1999. Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquaculture, 170: 267-283.
22. Hofer R., Kock G. 1989. Method for quantitative determination of digestive enzymes in fish larvae. Polish Archive Hydrobiology, 36: 439-441.
23. Hoseinifar H., Mirvaghefi A., Merrifield D.L. 2011. The effects of dietary inactive brewer’s yeast Saccharomyces siaecerevi var. ellipsoideus on the growth, physiological responses and gut microbiota of juvenile beluga (Huso huso). Aquaculture, 318: 90-94.
24. Kazemi R., Pourdehghani M., Yarmohammadi M., Yosefi Jourdehi A., Nasri Tajan, M. 2010. Cardiovascular System Physiology of Aquatic Animals and Applied Techniques of Fish Haematology. Bazargan Publicatio, Rasht, Iran. 194 P. (In Persian).
25. Kiffer J.D., Wakefield A.M., Litvak M.K. 2001. Juvenile exhibit reduced physiological response exercise. Journal of Experimental Biology, 204: 4281- 4289
26. Krogdahl A., Marie Bakke-McKellep A. 2005. Fasting and refeeding cause rapid changes in intestinal tissue mass and digestive enzyme capacities of Atlantic salmon (Salmo salar L.). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 141: 450-460.
27. Kuz’mina V., Shekovtsova N., Bolobonina V. 2010. Activity dynamics of proteinases and glycosidases of fish chyme with exposure in fresh and brackish water. Biology Bulletin, 37: 605-611.
28. Martinez F. H., Garcia-Riera M.P., Canteras M., De-costa J., Camora S. 1994. Blood Parameters in rainbow trout (Onchorhynchus mykiss): Smillatanneous influence of various factors. Comparative Biochemistry and Physiology, 101: 95-100
29. Martins M.L., Nomura D.T., Myiaazaki D.M.Y., Pilarshy F., Ribeiro K., Castro M.P., Campos CF.M. 2004. Physiological and haematological response of orochomis niloticus exposed to single and consecutive stress of capture. Acta Scientiarum, Animal Sciences, 26: 449-456.
30. Mohammadi Zarejabad A., Sudagar S., Pouralimotlagh S., Darvish Bastami K. 2010. Effects of rear temperature on hermatological and biochemical parameters of great sturgeon (Huso huso Linnaeus, 1758) juvenile. Comparative Clinical Pathology, 19: 367-371.
31. Mohseni M., Ozorio R.O.A., Pourkazemi M., Bai S.C. 2008. Effects of dietary Lcarnitine supplements on growth and body in beluga sturgeon (Huso huso) juveniles. Journal of Applied Ichthyology, 24(6): 646-649.
32. Nandeesha M.C., Gangadhar B., Keshavanath P., Varghese T.J. 2000. Effect of dietary sodium chloride supplementation on growth, biochemical composition and digestive enzyme activity of young Cyprinus carpio (Linn.) and Cirrhinus mrigala (Ham.). Journal of Aquaculture in the Tropics, 15: 135-144.
33. Nayak S.K. 2010. Probitics and immunity: a fish perspective. Fish and Shellfish Immunology, 29(1): 2-14.
34. Noori F., Van Stappen G., Sorgeloos P. 2011. Preliminary study on the activity of protease enzymes in Persian sturgeon (Acipenser persicus Borodin, 1897) larvae in response to different diets: effects on growth and survival. Aquaculture Research, 43: 198-207.
35. Nya E.J., Austin B. 2011. Dietary modulation of digestive enzymes by the administration of feed additives to rainbow trout, (Oncorhynchus mykiss) Walbaum. Aquaculture Nutrition, 17: 459-466.
36. Rubino V.C., Sanchez Vazquez F.J., Madrid J.A. 2005. Effect of salinity on food intake and macronutrient selection in European sea bass. Physiology and Behavior, 85(3): 333-339.
37. Salman N.A., Eddy F.B. 1988. Effects of dietary sodium chloride, on growth, food intake and conversion efficiency in rainbow trout (Salmo gairdneri, Richardson). Aquaculture 70: 131-144.
38. Senger H., Storch V., Reinecke M., Kloas W., Hanke W. 1994. The development of functional digestive and metabolic organs in turbot, Scophthalmus maximum. Marine Biology, 119: 471-486.
39. Taheri A., Abedian Kenari A., Hallaj R., Habibi Rezaei M., Motamed Zadegan A.S., Owji Fard U. 2011. Effect of different levels of hydrolysis protein on digestive enzymes of rainbow trout alvins (Oncorhynchus mykiss). Comparative Pathobiology, Scientific-Proceedings, 8(4): 674-665. (In Persian).
40. Thrall M.A., Baker D.C., Campbell T.W., Denicola, D., Fettman M.J., Lassen E.D., Re-bar A., Weiser G. 2004. Veterinary Hematology and Clinical Chemistry. Lippincott Williams and Wikins, USA. 618 P.
41. Tietz N.W. 1986. Texbook of Clinical Chemistry, W.B., Saunders, Philadelphia, USA. 579 P.
42. Ueberschar B. 1993. Measurement of Proteolytic Enzyme activity: significance and application in larval fish research. In: Walter BT, Fyhn HJ (Eds.). Physiological and biochemical aspects of fish development. University of Bergen, Norway, pp: 233-239.
43. Ugolev A.M, Kuz'mina V.V. 1994. Fish enterocyte hydrolases. Nutrition adaptations. Comparative Biochemistry and physiology, 107: 187-93.
44. Wahnon R., Coogan V., Mokady S. 1992. Dietary fish oil modulates the alkaline phosphatase activity and not the fluidity of rat intestinal microvillus membrane. Journal of Nutrition, 122(5): 1077-1084.
45. Worthington C.C. 1991. Worthington enzyme manual related Biochemical. 3rd Edition. Freehold, New Jersey, USA. 346 P.
46. Wu T., Sun L.C., Du C.H., Cai Q.F., Zhang Q.B., Su W.J., Cao M.J. 2009. Identification of pepsinogens and pepsins from the stomach of European eel (Anguilla anguilla). Food Chemistry, 115: 137-42.
47. Yeganeh S., Ramezanzadeh F., Jani-Khalili K., Babaei S.S. 2014. Investigating the effects of light period on the activity of some gastrointestinal and intestinal digestive enzymes in larvae and teenagers rainbow trout (Oncorhynchus mykiss). Iranian Journal of Fisheries Science, 23(2): 1-14. (In Persian).
48. Yooneszadeh M., Bahmani M., Kazemi R., Pourdehghani M., Feizbaksh H. 2009. The survey of seasonal changes of cortisol, Glucose and Ionic in Farmed female Stellate Sturgeon, Acipenser stellatus. Journal of Fisheries, 4: 1-11.
49. Yousefian M., Sheikholeslami Amiri M.., Kor D. 2011. Serum Biochemical Parameter of Male, Immature and Female Persian Sturgeon (Acipencer Persicus). Fisheries Journal, Islamic Azad University, Azadshahr Branch, 6(4): 9-14. (In Persian).
50. Yufera M., Darias M.J. 2007. The onset of exogenous feeding in marine fish larvae. Aquaculture, 268: 53-63.
51. Yúfera M., Fernández Díaz C., Vidaurreta A., Cara J.B., Moyano F.J. 2004. Gastrointestinal pH development of the acid digestion in larvae in earlyjuveniles of Sparus aurata (Pisces: Teleostei). Marine Biology 144(5): 863-869.
52. Zambonino-Infante J., Gisbert E., Sarasquete C., Navarro I., Gutiérrez J., Cahu C.L. 2009. Ontogeny and physiology of the digestive system of marine fish larvae. In: Cyrino JEO, Bureau D, Kapoor BG (Eds.). Feeding and Digestive Functions of Fish. Science Publishers, Incorporation, Enfield, USA, pp: 277- 344.
53. Zaugg W.S., Roley D.D., Prentice E.F., Gores K.X. 1983. Increased seawater survival and contribution to the fishery of Chinook salmon (Oncorhynchus tshawytscha) by supplemental dietary salt. Aquaculture, 32(1): 183-188.
54. Zhou F.J., Xu R., Ma J., Xu Z. 2010. Quantitative l-lysine requirement of juvenile black sea bream (Sparus macrocephalus). Aquaculture Nutrition, 16: 194-204.
55. Zoltowska K., Kolman R., Lopienska E., Kolman H. 1999. Activity of digestive enzymes in Siberian sturgeon juveniles (Acipenser baeri Brandt), a preliminary study. Archives of Polish Fisheries, 7: 201-211.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

hasanpor S, ghomi M, vahabzadeh rodsary H, Mousavi H, Kazemi R. Study on the activity of some digestive enzymes and biochemical parameters of blood effected by applying Nacl in the diet of farmed Beluga (Huso huso) juveniles. JAIR. 2019; 7 (2) :13-28
URL: http://jair.gonbad.ac.ir/article-1-619-en.html

Volume 7, Issue 2 (7-2019) Back to browse issues page
نشریه علمی پژوهشی پژوهشهای ماهی شناسی کاربردی Journal of Applied Ichthyological Research
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4144