Some 78 specimencs of trout barb (Capoeta trutta) from four rivers of Bushehr Basin were caught by seine net to compare their morphology using geometric morphometrics. The specimens after anestethizing in 1% clove oil, were fixed in bufferd 10% formalin and transferred to the Isfahan University of Technology Ichthyology Museum for further studies. Geometric morphometric method were applied to accomplish the biometric studies. For statistical analysis, Canonical Variable Analysis (CVA), Principle Component Analysis (PCA), Cluster Analysis (CA) and Discriminant Function Analysis (DFA) were employed. The PCA, CVA and CA showed differences between KheirAbad and Fahlian populations from Bushehr Basin in geometric morphometrics. This result showed that geometric morphometric methods could separate many populations of Bushehr Basin from each other. However, for more details, molecular studies are needed.