The present study aims at investigating hematological and biochemical parameters (electrolyte and non-electrolyte factors) in two species of Acipenseridae, A. persicus and H. huso among three different ages, fry (2-7 months), juvenile (1-2 years) and brood stocks (7years). The study was conducted in Caspian Sea Sturgeon International Research Institute in 2012 during six month (spring and summer). For this purpose, 30 A. persicus and 30 H. huso were used (10 fry fishes, 10 juvenile fishes, 5 female and 5 male brood stocks in each group). Comparison of means of hematological and biochemical parameters revealed a significant difference among the three groups regarding potassium ion in H. huso and MCH and monocytes in A. persicus in all the indices except for RBC, MCHC and glucose. (p<0/05). But in terms of sex, significant statistical difference was only observed in glucose and potassium ion in H. huso species and in albumin, chloride and sodium ions in A. persicus (p<0/05). By investigating the present results, it can be stated that age, species and sex (broodstocks) is the most effective in the change of biochemical and blood indices.