In this study, 236 Barbus lacerta specimens were collected (monthly) from three stations in Sefidroud river during eight months in order to survey some biological characteristics (December and March, 2011 and April to September 2012). The specimens ranged in size from 48 to 227 mm, total length and weighed from 1.03 to 113 g, total weight. The Average length of males and females was 108.39 ± 19.56 and 135.88 ± 32.31 mm, respectively, and mean weight 11.35 ± 6.13 and 25.33 ± 15.16 g. The Results of age determination by means of the gill cover bones (opercula) suggested that the maximum age found in females was 4+ and in males 3+. Also, the most abundant age groups were 2+ and 3+ for males and females, respectively. According to the results, the overall sex ratio of males to females was 1: 1.13 and there is no significant difference from the expected ratio of 1: 1 (P> 0.05). Length-weight relationships were estimated as W = 0.0054TL3.1628 for males, and W = 0.0036TL3.3244 for females implying that growth was positively allometric for both sexes. The von Bertalanffy growth function was estimated to be for males and for females. The greatest spontaneous growth observed in both sexes was between the ages of one and two and with an increase in age, this rate showed a significant decrease. In addition, gonadosomatic index showed that the reproduction period of this species in Sefidroud River occurred around April-June, with the peak of 7.03 ± 1.58 and 6.36 ± 2.40 in May for males and females, respectively.